Livzym Hemicellulase (Xylanase) – LIVZYM Biotechnology

Livzym enzymes · Baking

Hemicellulase (Xylanase)

A dough conditioner that works on the arabinoxylan fibers of flour — for extensible, machinable dough, superior gas retention and a finer crumb.

Livzym Hemicellulase is a food-grade xylanase produced through microbial fermentation. It hydrolyzes the β-xylosidic bonds of the arabinoxylan fibers naturally present in cereal flours, both refined and whole grain — the non-starch polysaccharides (NSPs) of baking flour — and is added in bread making to improve dough machinability, manageability and stability, bread quality and loaf volume.

Mode of action

Arabinoxylan is a chain of xylose units carrying arabinose side groups. Hemicellulase — an endo-β-(1,4)-D-xylanase — cuts that backbone at points along the chain, turning the long fibers into smaller arabinoxylan fragments. The smaller fragments bind far less water, so more of it becomes available to the gluten, and the fibers no longer get in the way of the gluten network.

Arabinoxylan (AX)xylan backbone with arabinose side groups
Hemicellulaseendo-β-(1,4)-D-xylanase cuts the xylan backbone
Shorter arabinoxylan chainsside groups still attached
Xylo-oligosaccharidestwo to three xylose units
Smaller fragments hold less water and interfere less with the gluten — the dough becomes more extensible and stable.

Features & benefits

Starting to work directly in mixing, Xylanase helps obtain the following characteristics in dough and bread.

  • Extensible dough, better pan flowHigher dough extensibility and increased pan flow.
  • Machinability and gas retentionIncreased dough machinability and superior gas retention for optimum oven spring and product volume.
  • A stronger gluten networkStronger and more continuous gluten network to minimize gas bubble coalescence.
  • A finer, more even crumbFiner and more even gas cell structure in the crumb.

Tested & approved

  1. Reference
  2. 3 ppm Market Benchmark
  3. 3 ppm UH 501
Baking trial: three stacks of three rolls, left to right the reference, 3 ppm market benchmark and 3 ppm UH 501, the rolls growing larger from left to right
Rolls: three per condition, from the untreated reference to 3 ppm of Hemicellulase UH 501.
  1. Reference
  2. 2 ppm Market Benchmark
  3. 1.5 ppm UH 501
  4. 2 ppm UH 501
Baking trial loaves, two per condition, left to right: reference, 2 ppm market benchmark, 1.5 ppm UH 501 and 2 ppm UH 501
Loaves: two per condition, from the untreated reference to 1.5 and 2 ppm of Hemicellulase UH 501.

Swipe sideways to see all four trials.

Livzym solution

Six grades with the same mode of action and application, from UH 20 to the high-activity UH 501 and UH 702 — choose by the activity your recipe needs.

Product Category Activity Dosage
Hemicellulase UH 20 Dough conditioning min. 620 XU/g —
Hemicellulase UH 26 Dough conditioning min. 1,000 XU/g 25–75 ppm
Hemicellulase UH 45 Dough conditioning min. 1,360 XU/g —
Hemicellulase UH 47 Dough conditioning min. 8,000 XU/g 3–20 ppm
Hemicellulase UH 501 Dough conditioning min. 20,000 XU/g 1–5 ppm
Hemicellulase UH 702 Dough conditioning min. 20,000 XU/g 1–5 ppm

XU: xylanase units. All grades are white-beige to off-white powders; store at max. 25 °C in a tightly closed package, away from direct sunlight and moisture, for a shelf life of 12 months.

  • Food-grade
  • Non-GMO
  • FSSC 22000
  • Halal
  • Kosher
  • ISO 9001 & 14001
  • JECFA / FCC purity

Activity profiles

Temperature

Relative activity of Livzym Hemicellulase versus temperatureRelative activity rises from about 27% at 30 °C to a maximum of 100% at about 62 °C, then falls to about 27% at 80 °C and 14% at 85 °C. over 80 % activity, 54–70 °C 0% 20% 40% 60% 80% 100% 120% 30 40 50 60 70 80 90 Temperature (°C) Relative activity (%) maximum near 62 °C 30 °C: 27% 35 °C: 33% 40 °C: 42% 45 °C: 53% 50 °C: 66% 55 °C: 86% 60 °C: 100% 65 °C: 97% 70 °C: 84% 75 °C: 57% 80 °C: 27% 85 °C: 14%

Maximum activity around 62 °C, with over 80% of activity between 54 and 70 °C; about 27% at 30 °C and at 80 °C.

pH

Relative activity of Livzym Hemicellulase versus pHRelative activity rises from about 6% at pH 2 to a maximum of 100% at pH 6, then falls to about 49% at pH 8 and 3% at pH 10. over 80 % activity, pH 5.2–6.9 0% 20% 40% 60% 80% 100% 120% 2 3 4 5 6 7 8 9 10 pH Relative activity (%) maximum at pH 6 pH 2: 6% pH 2.5: 15% pH 3: 25% pH 3.5: 37% pH 4: 49% pH 4.5: 62% pH 5: 76% pH 5.5: 91% pH 6: 100% pH 6.5: 93% pH 7: 79% pH 7.5: 64% pH 8: 49% pH 8.5: 33% pH 9: 18% pH 9.5: 10% pH 10: 3%

Maximum activity at pH 6, with over 80% of activity between pH 5.2 and 6.9 and more than half between pH 4 and 8.